RBM8A Required for pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Belongs to the RBM8A family. Ubiquitous. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: RNA splicing; RNA-binding; Spliceosome; Translation
Chromosomal Location of Human Ortholog: 3|3 F2.1
Cellular Component:  catalytic step 2 spliceosome; dendrite; exon-exon junction complex; neuronal cell body; nuclear speck; nucleus; U2-type catalytic step 1 spliceosome
Molecular Function:  mRNA binding
Biological Process:  mRNA splicing, via spliceosome; nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; regulation of alternative mRNA splicing, via spliceosome
Reference #:  Q9CWZ3 (UniProtKB)
Alt. Names/Synonyms: 2310057C03Rik; AA673428; Rbm; Rbm8; Rbm8a; Ribonucleoprotein RBM8A; RNA binding motif protein 8a; RNA-binding motif protein 8A; RNA-binding protein 8A
Gene Symbols: Rbm8a
Molecular weight: 19,889 Da
Basal Isoelectric point: 5.5  Predict pI for various phosphorylation states
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RBM8A

Protein Structure Not Found.


Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein