PLK3 Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.21; Kinase, protein; Nucleolus; Other group; PLK family; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of human Ortholog: 1p34.1
Cellular Component:  centrosome; cytoplasm; dendrite; Golgi stack; neuronal cell body; nucleolus; nucleoplasm; nucleus
Molecular Function:  ATP binding; p53 binding; protein binding; protein serine/threonine kinase activity
Biological Process:  apoptotic process; cellular response to DNA damage stimulus; cytoplasmic microtubule organization; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; endomitotic cell cycle; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; Golgi disassembly; mitotic cell cycle checkpoint; mitotic G1/S transition checkpoint; negative regulation of apoptotic process; negative regulation of transcription by RNA polymerase II; positive regulation of chaperone-mediated autophagy; positive regulation of intracellular protein transport; positive regulation of proteasomal ubiquitin-dependent protein catabolic process involved in cellular response to hypoxia; protein kinase B signaling; protein phosphorylation; regulation of cell division; regulation of cytokinesis; regulation of signal transduction by p53 class mediator; response to osmotic stress; response to radiation; response to reactive oxygen species
Reference #:  Q9H4B4 (UniProtKB)
Alt. Names/Synonyms: CNK; cytokine-inducible kinase; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; FNK; PLK-3; PLK3; polo like kinase 3; Polo-like kinase 3; polo-like kinase 3 (Drosophila); PRK; Proliferation-related kinase; Serine/threonine-protein kinase PLK3
Gene Symbols: PLK3
Molecular weight: 71,629 Da
Basal Isoelectric point: 9.28  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PLK3

Protein Structure Not Found.


Substrate Sequence Logo
Open Substrate Page

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  KinBase  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene