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RENT1
RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability. Belongs to the DNA2/NAM7 helicase family. Ubiquitous. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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| Protein type: C2H2-type zinc finger protein; EC 3.6.1.-; EC 3.6.4.-; Hydrolase; RNA-binding |
| Chromosomal Location of mouse Ortholog: 8 B3.3|8 34.15 cM |
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Cellular Component:
chromatin; chromosome, telomeric region; cytoplasm; cytosol; exon-exon junction complex; nucleoplasm; nucleus; supraspliceosomal complex
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Molecular Function:
ATP binding; ATP hydrolysis activity; chromatin binding; DNA binding; double-stranded DNA helicase activity; helicase activity; hydrolase activity; metal ion binding; nucleotide binding; protein binding; protein-containing complex binding; RNA binding; RNA helicase activity; telomeric DNA binding; zinc ion binding
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Biological Process:
3'-UTR-mediated mRNA destabilization; cell cycle phase transition; DNA duplex unwinding; DNA repair; DNA replication; histone mRNA catabolic process; nuclear-transcribed mRNA catabolic process; nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; positive regulation of mRNA catabolic process; regulation of telomere maintenance; regulation of translational termination; telomere maintenance via semi-conservative replication
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Reference #:
Q9EPU0
(UniProtKB)
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Alt. Names/Synonyms: ATP-dependent helicase RENT1; B430202H16Rik; mUpf1; Nonsense mRNA reducing factor 1; NORF1; PNO; PNORF-1; Regulator of nonsense transcripts 1; Regulator of nonsense transcripts 1 (Nonsense mRNA reducing factor 1) (NORF1) (Up-frameshift suppressor 1 homolog); Ren; Rent1; Up-frameshift suppressor 1 homolog; Upf1; UPF1 regulator of nonsense transcripts homolog (yeast); Upflp
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Gene Symbols: Upf1
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Molecular weight:
123,967 Da
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Basal Isoelectric point:
6.18
Predict pI for various phosphorylation states
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Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology®
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