RENT1 RNA-dependent helicase and ATPase required for nonsense- mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1- eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability. Found in a post-splicing messenger ribonucleoprotein (mRNP) complex. Associates with the exon junction complex (EJC). Associates with the SGM1C complex; is phosphorylated by the complex kinase component SGM1. Interacts with UPF2, UPF3A and UPF3B. Interacts with EST1A and SLBP. Interacts (when hyperphosphorylated) with PNRC2. Interacts with EIF2C1, EIF2C2 and GSPT2. Ubiquitous. Belongs to the DNA2/NAM7 helicase family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: C2H2-type zinc finger protein; EC 3.6.1.-; EC 3.6.4.-; Hydrolase; RNA-binding
Chromosomal Location of Human Ortholog: 19p13.11
Cellular Component:  chromatin; cytoplasm; cytosol; exon-exon junction complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus; P-body; supraspliceosomal complex
Molecular Function:  ATP binding; ATP-dependent RNA helicase activity; chromatin binding; helicase activity; protein binding; RNA binding; telomeric DNA binding; zinc ion binding
Biological Process:  3'-UTR-mediated mRNA destabilization; cell cycle phase transition; cellular response to interleukin-1; cellular response to lipopolysaccharide; DNA repair; DNA replication; dosage compensation by inactivation of X chromosome; histone mRNA catabolic process; mRNA export from nucleus; nuclear-transcribed mRNA catabolic process; nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay; nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; positive regulation of mRNA catabolic process; regulation of telomere maintenance; regulation of translational termination; telomere maintenance via semi-conservative replication; viral process
Reference #:  Q92900 (UniProtKB)
Alt. Names/Synonyms: ATP-dependent helicase RENT1; delta helicase; FLJ43809; FLJ46894; hUpf1; KIAA0221; Nonsense mRNA reducing factor 1; NORF1; pNORF1; Regulator of nonsense transcripts 1; RENT1; UP Frameshift 1; up-frameshift mutation 1 homolog; Up-frameshift suppressor 1 homolog; UPF1; UPF1 regulator of nonsense transcripts homolog (yeast); yeast Upf1p homolog
Gene Symbols: UPF1
Molecular weight: 124,345 Da
Basal Isoelectric point: 6.18  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

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