XPB ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. Belongs to the helicase family. RAD25/XPB subfamily. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; EC; Helicase; Transcription factor
Chromosomal Location of mouse Ortholog: 18|18 B1
Cellular Component:  nucleoplasm; nucleotide-excision repair factor 3 complex; nucleus; transcription factor TFIID complex; transcription factor TFIIH core complex; transcription factor TFIIH holo complex; transcription preinitiation complex
Molecular Function:  3'-5' DNA helicase activity; ATP binding; ATP hydrolysis activity; DNA binding; DNA helicase activity; helicase activity; hydrolase activity; nucleotide binding; promoter-specific chromatin binding
Biological Process:  apoptotic process; DNA damage response; DNA repair; DNA topological change; embryonic organ development; hair cell differentiation; nucleotide-excision repair; nucleotide-excision repair, DNA duplex unwinding; positive regulation of apoptotic process; protein localization; regulation of mitotic cell cycle phase transition; response to oxidative stress; response to UV; transcription by RNA polymerase II; transcription elongation by RNA polymerase I; transcription initiation at RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; UV protection
Reference #:  P49135 (UniProtKB)
Alt. Names/Synonyms: Basic transcription factor 2 89 kDa subunit; BTF2 p89; BTF2-p89; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; Ercc-3; Ercc3; excision repair 3; excision repair cross-complementing rodent repair deficiency, complementation group 3; General transcription and DNA repair factor IIH helicase subunit XPB; OTTMUSP00000028389; TFIIH 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH subunit XPB; Xeroderma pigmentosum group B-complementing protein; Xpb; Xpbc
Gene Symbols: Ercc3
Molecular weight: 89,126 Da
Basal Isoelectric point: 6.79  Predict pI for various phosphorylation states
CST Pathways:  Protein Acetylation
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  Reactome  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein