XPB ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. Belongs to the helicase family. RAD25/XPB subfamily. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; EC 3.6.4.12; Helicase; Transcription factor
Chromosomal Location of Human Ortholog: 2q14.3
Cellular Component:  nucleoplasm; nucleus; transcription factor TFIID complex; transcription factor TFIIH holo complex
Molecular Function:  3'-5' DNA helicase activity; ATP binding; ATPase activity; damaged DNA binding; DNA binding; helicase activity; protein binding; protein C-terminus binding; protein N-terminus binding; transcription factor binding
Biological Process:  7-methylguanosine mRNA capping; apoptotic process; DNA repair; DNA topological change; global genome nucleotide-excision repair; hair cell differentiation; nucleotide-excision repair; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; positive regulation of apoptotic process; positive regulation of transcription by RNA polymerase II; protein localization; protein phosphorylation; regulation of mitotic cell cycle phase transition; response to hypoxia; response to oxidative stress; response to UV; termination of RNA polymerase I transcription; transcription by RNA polymerase II; transcription elongation from RNA polymerase I promoter; transcription elongation from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; UV protection; viral process
Disease: Trichothiodystrophy 2, Photosensitive; Xeroderma Pigmentosum, Complementation Group B
Reference #:  P19447 (UniProtKB)
Alt. Names/Synonyms: Basic transcription factor 2 89 kDa subunit; BTF2; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair helicase; DNA repair protein complementing XP-B cells; ERCC excision repair 3, TFIIH core complex helicase subunit; ERCC3; excision repair cross-complementation group 3; excision repair cross-complementing rodent repair deficiency, complementation group 3; excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing); General transcription and DNA repair factor IIH helicase subunit XPB; GTF2H; RAD25; TFIIH; TFIIH 89 kDa subunit; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p89; TFIIH subunit XPB; TTD2; Xeroderma pigmentosum group B-complementing protein; xeroderma pigmentosum, complementation group B; XPB; XPBC
Gene Symbols: ERCC3
Molecular weight: 89,278 Da
Basal Isoelectric point: 6.83  Predict pI for various phosphorylation states
CST Pathways:  Protein Acetylation
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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XPB

Protein Structure Not Found.


Cross-references to other databases:  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene