PHF8 Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3. Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily. 5 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Demethylase; EC; Nucleolus
Chromosomal Location of Human Ortholog: X|X F3
Cellular Component:  nuclear membrane; nucleolus; nucleus
Molecular Function:  chromatin binding; histone demethylase activity; histone demethylase activity (H3-K27 specific); histone demethylase activity (H3-K36 specific); histone demethylase activity (H3-K9 specific); histone demethylase activity (H4-K20 specific); iron ion binding; methylated histone binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; zinc ion binding
Biological Process:  G1/S transition of mitotic cell cycle; histone H3-K27 demethylation; histone H3-K36 demethylation; histone H3-K9 demethylation; histone H4-K20 demethylation; negative regulation of chromatin silencing at rDNA; positive regulation of transcription by RNA polymerase I; positive regulation of transcription, DNA-templated
Reference #:  Q80TJ7 (UniProtKB)
Alt. Names/Synonyms: 9830141C09Rik; [histone H3]-dimethyl-L-lysine(36) demethylase PHF8; [histone H3]-dimethyl-L-lysine(9) demethylase PHF8; Histone lysine demethylase PHF8; Kiaa1111; mKIAA1111; OTTMUSP00000018735; PHD finger protein 8; Phf8
Gene Symbols: Phf8
Molecular weight: 113,553 Da
Basal Isoelectric point: 8.69  Predict pI for various phosphorylation states
CST Pathways:  Histone Methylation
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene