PLK1 a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Contains two polo-box domains (PBD), which are specific phosphoserine or -threonine binding domains. Acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Plays a key role in centrosome functions and the assembly of bipolar spindles. Promotes the targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Involved in mitosis exit and cytokinesis. Recruited at the central spindle by phosphorylating and docking PRC1 and KIF20A; creates its own docking sites on PRC1 and KIF20A by phosphorylating sites that are subsequently recognized by the PBDs. Phosphorylates RACGAP1, thereby creating a docking site for the Rho GTP exchange factor ECT2 that is essential for the cleavage furrow formation. Plays a central role in G2/M transition of mitotic cell cycle. Part of a regulatory circuit that promotes the activation of CDK1 by phosphorylating the positive regulator CDC25C and inhibiting the negative regulators WEE1 and MYT1. Involved in kinetochore functions and sister chromatid cohesion. Required for recovery after DNA damage checkpoint and entry into mitosis. Regulates nuclear envelope breakdown during prophase. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Note: This description may include information from UniProtKB.

Protein type: EC 2.7.11.21; Kinase, protein; Other group; PLK family; PLK1 subfamily; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of human Ortholog: 16p12.2

Cellular Component: centriolar satellite; centriole; centrosome; chromatin; cytoplasm; cytosol; kinetochore; microtubule cytoskeleton; midbody; mitotic spindle pole; nucleoplasm; nucleus; outer kinetochore; spindle; spindle microtubule; spindle midzone; spindle pole; synaptonemal complex

Molecular Function: anaphase-promoting complex binding; ATP binding; identical protein binding; magnesium ion binding; microtubule binding; protein binding; protein kinase activity; protein kinase binding; protein serine kinase activity; protein serine/threonine kinase activity

Biological Process: centrosome cycle; establishment of mitotic spindle orientation; establishment of protein localization; female meiosis chromosome segregation; G2/M transition of mitotic cell cycle; homologous chromosome segregation; microtubule bundle formation; mitotic cell cycle; mitotic chromosome condensation; mitotic cytokinesis; mitotic G2 DNA damage checkpoint signaling; mitotic nuclear membrane disassembly; mitotic sister chromatid segregation; mitotic spindle assembly checkpoint signaling; mitotic spindle organization; negative regulation of apoptotic process; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of transcription by RNA polymerase II; nuclear membrane disassembly; peptidyl-serine phosphorylation; positive regulation of peptidyl-threonine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein localization to nucleus; positive regulation of proteolysis; positive regulation of ubiquitin protein ligase activity; positive regulation of ubiquitin-protein transferase activity; protein destabilization; protein localization to chromatin; protein localization to nuclear envelope; protein phosphorylation; protein ubiquitination; regulation of anaphase-promoting complex-dependent catabolic process; regulation of cell cycle; regulation of cytokinesis; regulation of mitotic cell cycle; regulation of mitotic cell cycle phase transition; regulation of mitotic metaphase/anaphase transition; regulation of mitotic spindle assembly; regulation of protein binding; regulation of protein localization to cell cortex; sister chromatid cohesion; synaptonemal complex disassembly

Reference #: P53350 (UniProtKB)

Alt. Names/Synonyms: cell cycle regulated protein kinase; PLK; PLK-1; PLK1; polo (Drosophia)-like kinase; polo like kinase; polo like kinase 1; Polo-like kinase 1; polo-like kinase 1 (Drosophila); Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13

Gene Symbols: PLK1

Molecular weight: 68,255 Da

Basal Isoelectric point: 9.09 Predict pI for various phosphorylation states

CST Pathways: G2/M DNA Damage Checkpoint

Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology^®

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PLK1

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