PRAS40 iso3 Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component:  cytosol; nucleoplasm
Molecular Function:  protein binding
Biological Process:  negative regulation of cell size; negative regulation of protein kinase activity; negative regulation of TOR signaling; neurotrophin TRK receptor signaling pathway; regulation of apoptotic process; regulation of cellular response to heat; regulation of neuron apoptotic process
Reference #:  Q96B36-3 (UniProtKB)
Alt. Names/Synonyms: 40 kDa proline-rich AKT substrate; AKT1 substrate 1 (proline-rich); AKT1S1; AKTS1; Lobe; MGC2865; PRAS40; proline-rich Akt substrate, 40 kDa; Proline-rich AKT1 substrate 1
Gene Symbols: AKT1S1
Molecular weight: 29,262 Da
Basal Isoelectric point: 4.63  Predict pI for various phosphorylation states
CST Pathways:  Autophagy Signaling  |  Insulin Receptor Signaling  |  mTOR Signaling  |  PI3K/Akt Signaling  |  T Cell Receptor Signaling  |  Translation: eIF4E and p70S6K
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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PRAS40 iso3

Protein Structure Not Found.


Cross-references to other databases:  STRING  |  cBioPortal  |  Wikipedia  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein