RFFL E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling. Ubiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase; Vesicle
Chromosomal Location of human Ortholog: 17q12
Cellular Component:  cytoplasm; cytosol; endosome membrane; nucleoplasm; plasma membrane; recycling endosome membrane
Molecular Function:  metal ion binding; p53 binding; protease binding; protein binding; protein kinase binding; ubiquitin protein ligase activity; ubiquitin protein ligase binding
Biological Process:  apoptotic process; negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; negative regulation of signal transduction by p53 class mediator; negative regulation of tumor necrosis factor-mediated signaling pathway; proteasome-mediated ubiquitin-dependent protein catabolic process; protein K48-linked ubiquitination; regulation of fibroblast migration; regulation of TOR signaling; ubiquitin-dependent protein catabolic process
Reference #:  Q8WZ73 (UniProtKB)
Alt. Names/Synonyms: CARP-2; CARP2; caspase 8 and 10 associated RING protein-2; Caspase regulator CARP2; Caspases-8 and -10-associated RING finger protein 2; E3 ubiquitin-protein ligase rififylin; Fring; FYVE-RING finger protein Sakura; RFFL; RIFIFYLIN; ring finger and FYVE like domain containing E3 ubiquitin protein ligase; ring finger and FYVE-like domain containing 1; RING finger and FYVE-like domain-containing protein 1; RING finger protein 189; RING finger protein 34-like; RING-type E3 ubiquitin transferase rififylin; RNF189; RNF34L
Gene Symbols: RFFL
Molecular weight: 40,514 Da
Basal Isoelectric point: 5.33  Predict pI for various phosphorylation states
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene