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Raptor
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Interacts with MTOR. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity. Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Belongs to the WD repeat RAPTOR family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
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| Protein type: Adaptor/scaffold |
| Chromosomal Location of Human Ortholog: 17q25.3 |
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Cellular Component:
cytoplasm; cytoplasmic stress granule; cytosol; dendrite; lysosome; neuronal cell body; nucleoplasm; TORC1 complex
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Molecular Function:
14-3-3 protein binding; protein binding; protein binding, bridging; protein kinase activator activity; protein kinase binding; protein serine/threonine kinase inhibitor activity; protein-containing complex binding; RNA polymerase III type 1 promoter DNA binding; RNA polymerase III type 2 promoter DNA binding; RNA polymerase III type 3 promoter DNA binding; TFIIIC-class transcription factor complex binding
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Biological Process:
activation of protein kinase activity; cell cycle arrest; cellular response to amino acid stimulus; cellular response to leucine; cellular response to nutrient levels; negative regulation of protein serine/threonine kinase activity; positive regulation of cell growth; positive regulation of endothelial cell proliferation; positive regulation of G1/S transition of mitotic cell cycle; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-threonine phosphorylation; positive regulation of protein serine/threonine kinase activity; positive regulation of TOR signaling; positive regulation of transcription by RNA polymerase III; regulation of cell growth; regulation of cell size; regulation of cellular response to heat; regulation of macroautophagy; TOR signaling; TORC1 signaling; viral process
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Reference #:
Q8N122
(UniProtKB)
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Alt. Names/Synonyms: KIAA1303; KOG1; Mip1; p150 target of rapamycin (TOR)-scaffold protein; p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats; RAPTOR; regulatory associated protein of MTOR, complex 1; Regulatory-associated protein of mTOR; RPTOR
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Gene Symbols: RPTOR
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Molecular weight:
149,038 Da
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Basal Isoelectric point:
6.43
Predict pI for various phosphorylation states
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CST Pathways:
AMPK Signaling
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Autophagy Signaling
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Insulin Receptor Signaling
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mTOR Signaling
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PI3K/Akt Signaling
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T Cell Receptor Signaling
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Translation: eIF4E and p70S6K
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Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®
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