HUWE1 E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of isoform 2 of PA2G4. Acts in concert with MYCBP2 to regulate the circadian clock gene expression by promoting the lithium-induced ubiquination and degradation of NR1D1. Belongs to the UPL family. TOM1/PTR1 subfamily. Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.-; EC; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: Xp11.22
Cellular Component:  cytoplasm; cytosol; extracellular region; ficolin-1-rich granule lumen; nucleoplasm; nucleus; secretory granule lumen
Molecular Function:  DNA binding; protein binding; ubiquitin-protein transferase activity
Biological Process:  base-excision repair; cell differentiation; circadian regulation of gene expression; histone ubiquitination; neutrophil degranulation; positive regulation of protein ubiquitination; protein monoubiquitination; protein polyubiquitination
Disease: Mental Retardation, X-linked, Syndromic, Turner Type
Reference #:  Q7Z6Z7 (UniProtKB)
Alt. Names/Synonyms: ARF binding protein 1; ARF-binding protein 1; ARF-BP1; BJ-HCC-24 tumor antigen; E3 ubiquitin-protein ligase HUWE1; HECT domain protein LASU1; HECT, UBA and WWE domain containing 1; HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase; HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1; HECT, UBA and WWE domain-containing protein 1; HECT-type E3 ubiquitin transferase HUWE1; HectH9; Homologous to E6AP carboxyl terminus homologous protein 9; HSPC272; HUWE1; Ib772; KIAA0312; KIAA1578; Large structure of UREB1; LASU1; Mcl-1 ubiquitin ligase E3; MRXST; Mule; Upstream regulatory element-binding protein 1; URE-B1; URE-binding protein 1; UREB1
Gene Symbols: HUWE1
Molecular weight: 481,891 Da
Basal Isoelectric point: 5.1  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  Inhibition of Apoptosis  |  Mitochondrial Control of Apoptosis
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene