SPT5 Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. Belongs to the SPT5 family. Ubiquitously expressed. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: RNA-binding; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component:  DSIF complex; nucleoplasm; nucleus
Molecular Function:  chromatin binding; enzyme binding; protein binding; protein heterodimerization activity
Biological Process:  7-methylguanosine mRNA capping; negative regulation of DNA-templated transcription, elongation; negative regulation of mRNA polyadenylation; negative regulation of transcription by RNA polymerase II; positive regulation of DNA-templated transcription, elongation; positive regulation of macroautophagy; positive regulation of transcription by RNA polymerase II; positive regulation of viral transcription; transcription by RNA polymerase II; transcription elongation from RNA polymerase II promoter
Reference #:  O00267 (UniProtKB)
Alt. Names/Synonyms: DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; FLJ34157; hSPT5; SPT5; SPT5 homolog, DSIF elongation factor subunit; SPT5H; suppressor of Ty 5 homolog; suppressor of Ty 5 homolog (S. cerevisiae); SUPT5H; Tat-cotransactivator 1 protein; Tat-CT1; Tat-CT1 protein; Transcription elongation factor SPT5
Gene Symbols: SUPT5H
Molecular weight: 121,000 Da
Basal Isoelectric point: 4.95  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

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