PACT Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner. Belongs to the PRKRA family. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Activator; RNA processing; RNA-binding
Chromosomal Location of human Ortholog: 2q31.2
Cellular Component:  cytoplasm; cytosol; nucleoplasm; perinuclear region of cytoplasm; RISC-loading complex
Molecular Function:  double-stranded RNA binding; enzyme activator activity; enzyme binding; identical protein binding; pre-miRNA binding; protein binding; protein homodimerization activity
Biological Process:  cellular response to oxidative stress; immune response; middle ear morphogenesis; miRNA metabolic process; negative regulation of cell proliferation; outer ear morphogenesis; positive regulation of catalytic activity; positive regulation of intrinsic apoptotic signaling pathway; pre-miRNA processing; production of miRNAs involved in gene silencing by miRNA; production of siRNA involved in RNA interference; protein phosphorylation; protein stabilization; response to virus; skeletal system morphogenesis; viral process
Disease: Dystonia 16
Reference #:  O75569 (UniProtKB)
Alt. Names/Synonyms: DYT16; HSD14; Interferon-inducible double stranded RNA-dependent protein kinase activator A; Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PACT; PKR-associated protein X; PKR-associating protein X; PRKRA; protein activator of interferon induced protein kinase EIF2AK2; Protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA dependent activator; Protein kinase, interferon-inducible double stranded RNA-dependent activator; Protein kinase, interferon-inducible double-stranded RNA-dependent activator; RAX
Gene Symbols: PRKRA
Molecular weight: 34,404 Da
Basal Isoelectric point: 8.68  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein