POLA Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses. Belongs to the DNA polymerase type-B family. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; DNA replication; EC; Nuclear envelope; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase
Chromosomal Location of Human Ortholog: X C3|X 41.06 cM
Cellular Component:  alpha DNA polymerase:primase complex; cytoplasm; cytosol; nuclear envelope; nuclear matrix; nucleolus; nucleoplasm; nucleus
Molecular Function:  4 iron, 4 sulfur cluster binding; chromatin binding; DNA binding; DNA replication origin binding; DNA-directed DNA polymerase activity; double-stranded DNA binding; iron-sulfur cluster binding; metal ion binding; nucleic acid binding; nucleoside binding; nucleotide binding; nucleotidyltransferase activity; protein binding; protein heterodimerization activity; protein kinase binding; purine nucleotide binding; pyrimidine nucleotide binding; single-stranded DNA binding; transferase activity
Biological Process:  cell proliferation; DNA biosynthetic process; DNA replication; DNA replication initiation; DNA replication, synthesis of RNA primer; DNA strand elongation involved in DNA replication; DNA synthesis involved in DNA repair; double-strand break repair via nonhomologous end joining; lagging strand elongation; leading strand elongation; mitotic DNA replication initiation
Reference #:  P33609 (UniProtKB)
Alt. Names/Synonyms: AW321876; DNA polymerase alpha 1, 180 kDa; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DPOLA; G7a/Bat6Hom; OTTMUSP00000019499; Pola; Pola1; polymerase (DNA directed), alpha 1; polymerase, alpha 1
Gene Symbols: Pola1
Molecular weight: 167,340 Da
Basal Isoelectric point: 5.53  Predict pI for various phosphorylation states
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein