MSH6 Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-- Belongs to the DNA mismatch repair MutS family. 4 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: DNA-binding
Chromosomal Location of human Ortholog: 2p16.3
Cellular Component:  chromatin; cytosol; Golgi apparatus; intracellular membrane-bounded organelle; MutSalpha complex; nucleoplasm; nucleus
Molecular Function:  ADP binding; ATP binding; ATP-dependent activity, acting on DNA; ATP-dependent DNA damage sensor activity; chromatin binding; double-stranded DNA binding; enzyme binding; four-way junction DNA binding; guanine/thymine mispair binding; magnesium ion binding; methylated histone binding; mismatched DNA binding; MutLalpha complex binding; oxidized purine DNA binding; protein binding; protein homodimerization activity; single guanine insertion binding; single thymine insertion binding
Biological Process:  determination of adult lifespan; DNA repair; intrinsic apoptotic signaling pathway; intrinsic apoptotic signaling pathway in response to DNA damage; isotype switching; maintenance of DNA repeat elements; meiotic mismatch repair; mismatch repair; negative regulation of DNA recombination; positive regulation of helicase activity; response to UV; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments; spermatogenesis
Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 5; Endometrial Cancer; Mismatch Repair Cancer Syndrome 3
Reference #:  P52701 (UniProtKB)
Alt. Names/Synonyms: DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS homolog 6; mutS homolog 6 (E. coli); MutS protein homolog 6; MutS-alpha 160 kDa subunit; p160; sperm-associated protein
Gene Symbols: MSH6
Molecular weight: 152,786 Da
Basal Isoelectric point: 6.5  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

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