CDK4 Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: CDK family; CDK/CDK4 subfamily; CDK4 subfamily; CMGC group; Cell cycle regulation; EC; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of human Ortholog: 12q14.1
Cellular Component:  bicellular tight junction; chromatin; cyclin D1-CDK4 complex; cyclin D2-CDK4 complex; cyclin D3-CDK4 complex; cyclin-dependent protein kinase holoenzyme complex; cytosol; mediator complex; nuclear membrane; nucleolus; nucleoplasm; nucleus; transcription regulator complex
Molecular Function:  ATP binding; cyclin binding; cyclin-dependent protein serine/threonine kinase activity; cyclin-dependent protein serine/threonine kinase regulator activity; protein binding; protein serine kinase activity; RNA polymerase II CTD heptapeptide repeat kinase activity
Biological Process:  adipose tissue development; cell division; cellular response to insulin stimulus; cellular response to interleukin-4; cellular response to ionomycin; cellular response to lipopolysaccharide; cellular response to phorbol 13-acetate 12-myristate; G1/S transition of mitotic cell cycle; positive regulation of cell population proliferation; positive regulation of fibroblast proliferation; positive regulation of G2/M transition of mitotic cell cycle; protein phosphorylation; regulation of catalytic activity; regulation of cell cycle; regulation of gene expression; regulation of insulin receptor signaling pathway; regulation of lipid biosynthetic process; regulation of lipid catabolic process; regulation of multicellular organism growth; regulation of transcription initiation by RNA polymerase II; regulation of type B pancreatic cell proliferation; response to xenobiotic stimulus; signal transduction
Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 3
Reference #:  P11802 (UniProtKB)
Alt. Names/Synonyms: CDK4; cell division kinase 4; Cell division protein kinase 4; CMM3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; melanoma cutaneous malignant, 3; MGC14458; PSK-J3
Gene Symbols: CDK4
Molecular weight: 33,730 Da
Basal Isoelectric point: 6.52  Predict pI for various phosphorylation states
CST Pathways:  G1/S Checkpoint
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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