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RPS3
Involved in translation as a component of the 40S small ribosomal subunit. Has endonuclease activity and plays a role in repair of damaged DNA. Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA. Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS). Has also been shown to bind with similar affinity to intact and damaged DNA. Stimulates the N-glycosylase activity of the base excision protein OGG1. Enhances the uracil excision activity of UNG1. Also stimulates the cleavage of the phosphodiester backbone by APEX1. When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage. Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide. Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes. Represses its own translation by binding to its cognate mRNA. Binds to and protects TP53/p53 from MDM2-mediated ubiquitination. Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization. Involved in induction of apoptosis through its role in activation of CASP8. Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5. Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation. Belongs to the universal ribosomal protein uS3 family. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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| Protein type: Apoptosis; EC 4.2.99.18; RNA-binding; Ribosomal; Translation |
| Chromosomal Location of human Ortholog: 11q13.4 |
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Cellular Component:
cytoplasm; cytosol; cytosolic ribosome; cytosolic small ribosomal subunit; endoplasmic reticulum; mitochondrial inner membrane; mitochondrial matrix; mitotic spindle; NF-kappaB complex; nucleolus; nucleoplasm; nucleus; plasma membrane; polysome; postsynaptic density; ribonucleoprotein complex; ribosome; ruffle membrane
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Molecular Function:
class I DNA-(apurinic or apyrimidinic site) endonuclease activity; damaged DNA binding; DNA binding; DNA endonuclease activity; DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) endonuclease activity; DNA-binding transcription factor binding; enzyme binding; Hsp70 protein binding; Hsp90 protein binding; iron-sulfur cluster binding; kinase binding; microtubule binding; mRNA binding; oxidized purine DNA binding; oxidized pyrimidine DNA binding; protein binding; protein kinase A binding; protein kinase binding; protein-containing complex binding; RNA binding; RNA polymerase II transcription regulatory region sequence-specific DNA binding; small ribosomal subunit rRNA binding; structural constituent of ribosome; supercoiled DNA binding; tubulin binding; ubiquitin-like protein conjugating enzyme binding
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Biological Process:
apoptotic process; base-excision repair; cell division; cellular response to hydrogen peroxide; cellular response to reactive oxygen species; cellular response to tumor necrosis factor; chromosome segregation; cytoplasmic translation; DNA damage response; DNA repair; negative regulation of DNA repair; negative regulation of protein ubiquitination; negative regulation of translation; positive regulation of activated T cell proliferation; positive regulation of apoptotic signaling pathway; positive regulation of base-excision repair; positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis; positive regulation of DNA repair; positive regulation of endodeoxyribonuclease activity; positive regulation of gene expression; positive regulation of interleukin-2 production; positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage; positive regulation of JUN kinase activity; positive regulation of microtubule polymerization; positive regulation of NF-kappaB transcription factor activity; positive regulation of NIK/NF-kappaB signaling; positive regulation of protein-containing complex assembly; positive regulation of T cell receptor signaling pathway; regulation of apoptotic process; response to TNF agonist; spindle assembly; translation; translational initiation
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Reference #:
P23396
(UniProtKB)
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Alt. Names/Synonyms: 40S ribosomal protein S3; FLJ26283; FLJ27450; IMR-90 ribosomal protein S3; MGC87870; ribosomal protein S3; RPS3; RS3; S3; Small ribosomal subunit protein uS3
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Gene Symbols: RPS3
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Molecular weight:
26,688 Da
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Basal Isoelectric point:
9.68
Predict pI for various phosphorylation states
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Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology®
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