POLA Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses. Belongs to the DNA polymerase type-B family. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; DNA replication; EC; Nuclear envelope; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase
Chromosomal Location of human Ortholog: Xp22.11-p21.3
Cellular Component:  alpha DNA polymerase:primase complex; cytosol; nuclear envelope; nuclear matrix; nucleolus; nucleoplasm; nucleus
Molecular Function:  chromatin binding; DNA binding; DNA replication origin binding; DNA-directed DNA polymerase activity; nucleotide binding; protein binding; protein kinase binding; single-stranded DNA binding; zinc ion binding
Biological Process:  DNA repair; DNA replication; DNA replication initiation; DNA replication, synthesis of RNA primer; DNA strand elongation involved in DNA replication; DNA synthesis involved in DNA repair; DNA synthesis involved in UV-damage excision repair; double-strand break repair via nonhomologous end joining; lagging strand elongation; leading strand elongation; mitotic DNA replication initiation; nucleotide-excision repair; regulation of type I interferon production
Disease: Pigmentary Disorder, Reticulate, With Systemic Manifestations, X-linked; Van Esch-o'driscoll Syndrome
Reference #:  P09884 (UniProtKB)
Alt. Names/Synonyms: DKFZp686K1672; DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha 1, catalytic subunit; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; DPOLA; NSX; p180; POLA; POLA1; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD); VEODS
Gene Symbols: POLA1
Molecular weight: 165,913 Da
Basal Isoelectric point: 5.61  Predict pI for various phosphorylation states
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein