ACTN4 F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA. Belongs to the alpha-actinin family. Widely expressed. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of rat Ortholog: 1q21
Cellular Component:  brush border; cell-cell junction; cortical cytoskeleton; cytoplasm; cytosol; neuron projection; nuclear body; nucleus; perinuclear region of cytoplasm; protein-containing complex; pseudopodium; ribonucleoprotein complex; stress fiber; Z disc
Molecular Function:  actin binding; actin filament binding; calcium ion binding; chromatin DNA binding; ion channel binding; nuclear hormone receptor binding; nuclear receptor transcription coactivator activity; nucleoside binding; protein binding; protein homodimerization activity; protein N-terminus binding; protein-containing complex binding; retinoic acid receptor binding; RNA polymerase II regulatory region sequence-specific DNA binding; transcription coactivator activity
Biological Process:  actin filament bundle assembly; bicellular tight junction assembly; negative regulation of cellular component movement; negative regulation of substrate adhesion-dependent cell spreading; peroxisome proliferator activated receptor signaling pathway; positive regulation of cell migration; positive regulation of cellular component movement; positive regulation of NIK/NF-kappaB signaling; positive regulation of nucleic acid-templated transcription; positive regulation of pinocytosis; protein localization to bicellular tight junction; protein transport; regulation of apoptotic process; regulation of nucleic acid-templated transcription; response to hypoxia; retinoic acid receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; vesicle transport along actin filament
Reference #:  Q9QXQ0 (UniProtKB)
Alt. Names/Synonyms: actinin alpha 4; Actn4; alpha actinin 4; Alpha-actinin-4; Non-muscle alpha-actinin 4
Gene Symbols: Actn4
Molecular weight: 104,915 Da
Basal Isoelectric point: 5.27  Predict pI for various phosphorylation states
CST Pathways:  T Cell Receptor Signaling
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  Reactome  |  BioGPS  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene