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SF2
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. Belongs to the splicing factor SR family. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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| Protein type: RNA splicing; RNA-binding; Spliceosome |
| Chromosomal Location of mouse Ortholog: 11 C|11 52.4 cM |
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Cellular Component:
catalytic step 2 spliceosome; cytoplasm; exon-exon junction complex; nuclear speck; nucleoplasm; nucleus; spliceosomal complex
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Molecular Function:
DNA topoisomerase binding; molecular function regulator activity; mRNA binding; nucleic acid binding; protein binding; protein kinase B binding; RNA binding; RS domain binding
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Biological Process:
alternative mRNA splicing, via spliceosome; cardiac muscle contraction; cellular response to interleukin-17; in utero embryonic development; interleukin-17-mediated signaling pathway; mRNA 5'-splice site recognition; mRNA processing; mRNA stabilization; mRNA transport; positive regulation of RNA splicing; protein localization to nucleus; protein localization to P-body; regulation of RNA splicing; RNA splicing; signal transduction involved in regulation of gene expression
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Reference #:
Q6PDM2
(UniProtKB)
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Alt. Names/Synonyms: 1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; Asf; ASF/SF2; AW491331; OTTMUSP00000001418; Pre-mRNA-splicing factor SRp30a; serine and arginine-rich splicing factor 1; Serine/arginine-rich splicing factor 1; Sf2; Sfrs1; Splicing factor, arginine/serine-rich 1; splicing factor, arginine/serine-rich 1 (ASF/SF2); SRSF1
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Gene Symbols: Srsf1
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Molecular weight:
27,745 Da
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Basal Isoelectric point:
10.37
Predict pI for various phosphorylation states
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Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology®
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