CASP8 Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Belongs to the peptidase C14A family. Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. 9 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Apoptosis; EC; Protease
Chromosomal Location of Human Ortholog: 9q31
Cellular Component:  CD95 death-inducing signaling complex; cell body; cytoplasm; cytosol; death-inducing signaling complex; membrane raft; neuron projection; Noc1p-Noc2p complex; nucleoplasm; nucleus; plasma membrane; protein-containing complex; ripoptosome
Molecular Function:  cysteine-type endopeptidase activity; cysteine-type endopeptidase activity involved in apoptotic process; cysteine-type endopeptidase activity involved in apoptotic signaling pathway; death effector domain binding; death receptor binding; endopeptidase activity; identical protein binding; peptidase activity; protein binding; protein heterodimerization activity; protein-containing complex binding; scaffold protein binding; tumor necrosis factor receptor binding; ubiquitin protein ligase binding
Biological Process:  activation of cysteine-type endopeptidase activity; activation of cysteine-type endopeptidase activity involved in apoptotic process; angiogenesis; apoptotic process; apoptotic signaling pathway; cellular response to mechanical stimulus; cellular response to organic cyclic compound; execution phase of apoptosis; extrinsic apoptotic signaling pathway; extrinsic apoptotic signaling pathway via death domain receptors; heart development; hepatocyte apoptotic process; macrophage differentiation; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of necroptotic process; neural tube formation; positive regulation of apoptotic process; positive regulation of extrinsic apoptotic signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of macrophage differentiation; positive regulation of neuron death; positive regulation of proteolysis; proteolysis; proteolysis involved in cellular protein catabolic process; regulation of apoptotic signaling pathway; regulation of thymocyte apoptotic process; response to antibiotic; response to auditory stimulus; response to cobalt ion; response to cold; response to estradiol; response to ethanol; response to lipopolysaccharide; response to tumor necrosis factor; TRAIL-activated apoptotic signaling pathway
Reference #:  Q9JHX4 (UniProtKB)
Alt. Names/Synonyms: CASP8; caspase 8; Caspase-8; caspase8
Gene Symbols: Casp8
Molecular weight: 55,339 Da
Basal Isoelectric point: 5.28  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  Death Receptor Signaling  |  ErbB/HER Signaling  |  Inhibition of Apoptosis  |  Mitochondrial Control of Apoptosis  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene