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WASHC2C
Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes inhibits WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization and is involved in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission. Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1. Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35. Required for the endosomal recruitment of CCC and retriever complexes subunits COMMD1 and CCDC93 as well as the retrievere complex subunit VPS35L. (Microbial infection) Plays a role in fluid-phase endocytosis, a process exploited by vaccinia intracellular mature virus (IMV) to enter cells. As a result, may facilitate the penetration of IMV into cells. Belongs to the FAM21 family. 6 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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| Protein type: Unknown function |
| Chromosomal Location of human Ortholog: 10q11.22 |
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Cellular Component:
cytosol; early endosome; early endosome membrane; endosome; intracellular membrane-bounded organelle; nucleolus; plasma membrane; WASH complex
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Molecular Function:
phosphatidylinositol phosphate binding; phosphatidylinositol-3,4,5-trisphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-3,5-bisphosphate binding; phosphatidylinositol-3-phosphate binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-4-phosphate binding; phosphatidylinositol-5-phosphate binding; protein binding; retromer complex binding
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Biological Process:
endocytic recycling; endosomal transport; negative regulation of barbed-end actin filament capping; protein localization to endosome; protein transport; regulation of Arp2/3 complex-mediated actin nucleation; regulation of substrate adhesion-dependent cell spreading; retrograde transport, endosome to Golgi
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Reference #:
Q9Y4E1
(UniProtKB)
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Alt. Names/Synonyms: FA21C; FAM21A; FAM21C; family with sequence similarity 21 member C; family with sequence similarity 21, member C; KIAA0592; vaccinia virus penetration factor; VPEF; WAC2C; WASH complex subunit 2C; WASH complex subunit FAM21C; WASHC2C
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Gene Symbols: WASHC2C
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Molecular weight:
147,154 Da
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Basal Isoelectric point:
4.69
Predict pI for various phosphorylation states
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