NDST3 Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity. Belongs to the sulfotransferase 1 family. NDST subfamily. Expressed in brain, kidney, liver, fetal and adult lung, adult pancreas, placenta, fetal spleen and fetal thymus. Not detected in adult/ fetal heart and skeletal muscle. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC; Glycan Metabolism - heparan sulfate biosynthesis; Hydrolase; Membrane protein, integral; Transferase
Chromosomal Location of mouse Ortholog: 3|3 G1
Cellular Component:  Golgi apparatus; integral component of membrane; membrane
Molecular Function:  [heparan sulfate]-glucosamine N-sulfotransferase activity; catalytic activity; deacetylase activity; heparan sulfate N-acetylglucosaminyltransferase activity; heparan sulfate sulfotransferase activity; hydrolase activity; sulfotransferase activity; transferase activity
Biological Process:  heparan sulfate proteoglycan biosynthetic process; heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; metabolic process
Reference #:  Q9EQH7 (UniProtKB)
Alt. Names/Synonyms: 4921531K01Rik; 4930511P15Rik; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3; Glucosaminyl N-deacetylase/N-sulfotransferase 3; Heparan sulfate N-deacetylase 3; Heparan sulfate N-sulfotransferase 3; Hsst3; MGC90637; N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3; N-deacetylase/N-sulfotransferase 3; N-heparan sulfate sulfotransferase 3; N-HSST 3; NDST-3; Ndst3; OTTMUSP00000030393
Gene Symbols: Ndst3
Molecular weight: 101,040 Da
Basal Isoelectric point: 8.33  Predict pI for various phosphorylation states
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  Reactome  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene