EPRS Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. The phosphorylation of EPRS, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS function from protein synthesis to translation inhibition. Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan. In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family. Note: This description may include information from UniProtKB.
Protein type: Aminoacyl-tRNA synthetase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC; EC; Ligase; Translation; Translation regulation
Chromosomal Location of human Ortholog: 1q41
Cellular Component:  aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; GAIT complex; plasma membrane; ribonucleoprotein complex
Molecular Function:  ATP binding; glutamate-tRNA ligase activity; GTPase binding; identical protein binding; proline-tRNA ligase activity; protein binding; protein homodimerization activity; RNA stem-loop binding; zinc ion binding
Biological Process:  cellular response to insulin stimulus; cellular response to type II interferon; glutamyl-tRNA aminoacylation; negative regulation of translation; prolyl-tRNA aminoacylation; protein-containing complex assembly; regulation of long-chain fatty acid import into cell; tRNA aminoacylation for protein translation
Disease: Leukodystrophy, Hypomyelinating, 15
Reference #:  P07814 (UniProtKB)
Alt. Names/Synonyms: Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline--tRNA ligase; Cell proliferation-inducing gene 32 protein; DKFZp313B047; EARS; EPRS; EPRS1; GLNS; GLUPRORS; GluRS; glutamate tRNA ligase; Glutamate--tRNA ligase; Glutamatyl-prolyl-tRNA synthetase; glutaminyl-tRNA synthetase; glutamyl-prolyl-tRNA synthetase; glutamyl-prolyl-tRNA synthetase 1; Glutamyl-tRNA synthetase; HLD15; PARS; PIG32; proliferation-inducing gene 32 protein; proliferation-inducing protein 32; proline tRNA ligase; Proline--tRNA ligase; proline-tRNA ligase; Prolyl-tRNA synthetase; QARS; QPRS; SYEP
Gene Symbols: EPRS1
Molecular weight: 170,591 Da
Basal Isoelectric point: 7.02  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein