PU.1 Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing. Belongs to the ETS family. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: DNA-binding; Oncoprotein; Transcription factor
Chromosomal Location of mouse Ortholog: 2 E1|2 50.44 cM
Cellular Component:  nuclear chromatin; nucleoplasm; nucleus; transcription factor complex
Molecular Function:  DNA binding; DNA-binding transcription activator activity, RNA polymerase II-specific; DNA-binding transcription factor activity; DNA-binding transcription factor activity, RNA polymerase II-specific; DNA-binding transcription repressor activity, RNA polymerase II-specific; NFAT protein binding; protein binding; RNA binding; RNA polymerase II distal enhancer sequence-specific DNA binding; RNA polymerase II proximal promoter sequence-specific DNA binding; RNA polymerase II transcription factor binding; sequence-specific DNA binding; transcription factor binding
Biological Process:  anatomical structure regression; apoptotic process involved in blood vessel morphogenesis; cell differentiation; erythrocyte differentiation; granulocyte differentiation; histone H3 acetylation; hypermethylation of CpG island; interleukin-6-mediated signaling pathway; lymphocyte differentiation; lymphoid progenitor cell differentiation; macrophage differentiation; myeloid dendritic cell differentiation; myeloid leukocyte differentiation; negative regulation of gene expression, epigenetic; negative regulation of histone H4 acetylation; negative regulation of MHC class II biosynthetic process; negative regulation of transcription by RNA polymerase II; negative regulation of transcription, DNA-templated; positive regulation of pri-miRNA transcription by RNA polymerase II; positive regulation of transcription by RNA polymerase II; positive regulation of transcription, DNA-templated; pri-miRNA transcription by RNA polymerase II; regulation of erythrocyte differentiation; regulation of transcription by RNA polymerase II; regulation of transcription, DNA-templated; somatic stem cell population maintenance; vasculature development
Reference #:  P17433 (UniProtKB)
Alt. Names/Synonyms: 31 kDa-transforming protein; Dis; Dis-1; Dis1; hematopoietic transcription factor PU.1; OTTMUSP00000015228; PU.; PU.1; SFFV proviral integration 1; SFFV proviral integration 1 protein; Sfp; Sfpi-1; Sfpi1; Spi-1; Spi1; spleen focus forming virus (SFFV) proviral integration oncogene; Tcfpu; Tcfpu1; Tfpu.; Tfpu.1; Transcription factor PU.1
Gene Symbols: Spi1
Molecular weight: 31,349 Da
Basal Isoelectric point: 6.34  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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PU.1

Protein Structure Not Found.


Cross-references to other databases:  AlphaFold  |  STRING  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene