p53 a ubiquitous transcription factor and major tumor suppressor in many tumor types. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation at multiple sites, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylation by many kinases including Chk2 and Chk1 enhances its tetramerization, stability and activity. The phosphorylation by CAK increases in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53.The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. Nine human isoforms generated by alternative promoter usage or alternative splicing have been reported. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine. Belongs to the p53 family. Note: This description may include information from UniProtKB.
Protein type: Activator; DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription factor; Tumor suppressor
Chromosomal Location of Human Ortholog: 11 B3|11 42.83 cM
Cellular Component:  chromatin; cytoplasm; cytosol; endoplasmic reticulum; mitochondrial matrix; mitochondrion; nuclear chromatin; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML body; protein-containing complex; replication fork; site of double-strand break; transcription factor complex
Molecular Function:  ATP binding; chaperone binding; chromatin binding; copper ion binding; core promoter sequence-specific DNA binding; disordered domain specific binding; DNA binding; DNA-binding transcription activator activity, RNA polymerase II-specific; DNA-binding transcription factor activity; DNA-binding transcription factor activity, RNA polymerase II-specific; enzyme binding; histone acetyltransferase binding; histone deacetylase binding; histone deacetylase regulator activity; identical protein binding; MDM2/MDM4 family protein binding; metal ion binding; mRNA 3'-UTR binding; p53 binding; promoter-specific chromatin binding; protease binding; protein binding; protein C-terminus binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; protein phosphatase 2A binding; protein phosphatase binding; protein self-association; receptor tyrosine kinase binding; RNA polymerase II distal enhancer sequence-specific DNA binding; RNA polymerase II proximal promoter sequence-specific DNA binding; RNA polymerase II regulatory region sequence-specific DNA binding; RNA polymerase II transcription factor binding; sequence-specific DNA binding; TFIID-class transcription factor complex binding; transcription cofactor binding; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding
Biological Process:  apoptotic process; autophagy; B cell lineage commitment; bone marrow development; cardiac septum morphogenesis; cell aging; cell cycle; cell cycle arrest; cellular protein localization; cellular response to actinomycin D; cellular response to DNA damage stimulus; cellular response to gamma radiation; cellular response to glucose starvation; cellular response to ionizing radiation; cellular response to reactive oxygen species; cellular response to UV; cellular response to UV-C; central nervous system development; cerebellum development; chromatin assembly; chromosome organization; circadian behavior; circadian rhythm; determination of adult lifespan; DNA damage response, signal transduction by p53 class mediator; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; DNA strand renaturation; double-strand break repair; embryo development ending in birth or egg hatching; embryonic organ development; entrainment of circadian clock by photoperiod; ER overload response; gastrulation; heart development; hematopoietic progenitor cell differentiation; hematopoietic stem cell differentiation; in utero embryonic development; interferon-gamma-mediated signaling pathway; intrinsic apoptotic signaling pathway by p53 class mediator; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; intrinsic apoptotic signaling pathway in response to hypoxia; mitochondrial DNA repair; mitotic cell cycle arrest; mitotic G1 DNA damage checkpoint; mRNA transcription; multicellular organism development; multicellular organism growth; necroptotic process; negative regulation of apoptotic process; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of DNA biosynthetic process; negative regulation of DNA replication; negative regulation of fibroblast proliferation; negative regulation of gene expression; negative regulation of glucose catabolic process to lactate via pyruvate; negative regulation of mitophagy; negative regulation of mitotic cell cycle; negative regulation of neuroblast proliferation; negative regulation of production of miRNAs involved in gene silencing by miRNA; negative regulation of proteolysis; negative regulation of reactive oxygen species metabolic process; negative regulation of smooth muscle cell proliferation; negative regulation of telomerase activity; negative regulation of transcription by RNA polymerase II; negative regulation of transcription, DNA-templated; negative regulation of transforming growth factor beta receptor signaling pathway; neuron apoptotic process; nucleotide-excision repair; oligodendrocyte apoptotic process; oxidative stress-induced premature senescence; positive regulation of apoptotic process; positive regulation of cardiac muscle cell apoptotic process; positive regulation of cell aging; positive regulation of cell cycle; positive regulation of cell cycle arrest; positive regulation of execution phase of apoptosis; positive regulation of gene expression; positive regulation of histone deacetylation; positive regulation of intrinsic apoptotic signaling pathway; positive regulation of leukocyte migration; positive regulation of mitochondrial membrane permeability; positive regulation of neuron apoptotic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of pri-miRNA transcription by RNA polymerase II; positive regulation of production of miRNAs involved in gene silencing by miRNA; positive regulation of protein oligomerization; positive regulation of reactive oxygen species metabolic process; positive regulation of release of cytochrome c from mitochondria; positive regulation of RNA polymerase II transcriptional preinitiation complex assembly; positive regulation of thymocyte apoptotic process; positive regulation of transcription by RNA polymerase II; positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress; positive regulation of transcription from RNA polymerase II promoter in response to hypoxia; positive regulation of transcription from RNA polymerase II promoter in response to stress; positive regulation of transcription, DNA-templated; programmed cell death; protein homotetramerization; protein import into nucleus; protein localization; protein stabilization; protein tetramerization; protein-containing complex assembly; regulation of apoptotic process; regulation of cell cycle; regulation of cell proliferation; regulation of cellular senescence; regulation of DNA damage response, signal transduction by p53 class mediator; regulation of fibroblast apoptotic process; regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter; regulation of hydrogen peroxide-induced cell death; regulation of intracellular pH; regulation of intrinsic apoptotic signaling pathway by p53 class mediator; regulation of mitochondrial membrane permeability involved in apoptotic process; regulation of neuron apoptotic process; regulation of thymocyte apoptotic process; regulation of tissue remodeling; regulation of transcription by RNA polymerase II; regulation of transcription, DNA-templated; release of cytochrome c from mitochondria; replicative senescence; response to drug; response to gamma radiation; response to ischemia; response to oxidative stress; response to salt stress; response to UV; response to X-ray; rhythmic process; RNA polymerase II preinitiation complex assembly; rRNA transcription; signal transduction by p53 class mediator; somitogenesis; T cell differentiation in thymus; T cell lineage commitment; T cell proliferation involved in immune response; transforming growth factor beta receptor signaling pathway; viral process
Reference #:  P02340 (UniProtKB)
Alt. Names/Synonyms: bbl; bfy; bhy; Cellular tumor antigen p53; ME-delta123p53; mutant p53; p44; P53; p53 cellular tumor antigen; RP23-56I20.1; Tp53; transformation related protein 53; Trp53; Tumor suppressor p53; tumor supressor p53
Gene Symbols: Tp53
Molecular weight: 43,458 Da
Basal Isoelectric point: 6.83  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  Apoptosis Regulation  |  ErbB/HER Signaling  |  G1/S Checkpoint  |  G2/M DNA Damage Checkpoint  |  Mitochondrial Control of Apoptosis  |  PI3K/Akt Signaling  |  Protein Acetylation  |  Regulation of P38 MAPKs  |  SAPK/JNK Signaling Cascades  |  Warburg Effect
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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p53

Protein Structure Not Found.


Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene  |  NURSA  |  Ensembl Protein