CUL3 a core component of multiple cullin-RING-based BCR (BTB- CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with CAND1. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. SPOP is involved in ubiquitination of BMI1, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. BCR(KLHL12) is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31A or -B. BCR(KLHL3) acts as a regulator of ion transport in the distal nephron; possibly by mediating ubiquitination of SLC12A3/NCC. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Forms neddylation-dependent homodimers. Component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes formed of CUL3, RBX1 and a variable BTB domain-containing protein acting as both, adapter to cullin and substrate recognition subunit. The BCR complex may be active as a heterodimeric complex, in which NEDD8, covalently attached to one CUL3 molecule, binds to the C-terminus of a second CUL3 molecule. Interacts with RBX1, RNF7, CYCE and CAND1. Part of the BCR(SPOP) containing SPOP. Part of the probable BCR(KLHL9-KLHL13) complex with BTB domain proteins KLHL9 and KLHL13. Part of the BCR(KBTBD10) complex containing KBTBD10. Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. Component of the BCR(KLHL3) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL3 and RBX1 (Probable). Part of the BCR(ENC1) complex containing ENC1. Part of a complex consisting of BMI1, CUL3 and SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with KCTD5, KLHL9, KLHL13, GAN, ZBTB16, KLHL21, KLHL3, KLHL15, KLHL20, C16orf44, GMCL1P1, BTBD1. Part of a complex that contains CUL3, RBX1 and GAN. Interacts (via BTB domain) with KLHL17; the interaction regulates surface GRIK2 expression. Widely expressed. Belongs to the cullin family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of mouse Ortholog: 1|1 C5
Cellular Component:  cell projection; centrosome; cilium; Cul3-RING ubiquitin ligase complex; cullin-RING ubiquitin ligase complex; cytoplasm; cytoskeleton; glutamatergic synapse; Golgi apparatus; mitotic spindle; motile cilium; nucleus; plasma membrane; polar microtubule; sperm flagellum; spindle pole
Molecular Function:  cyclin binding; identical protein binding; Notch binding; POZ domain binding; protein binding; ubiquitin protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein transferase activity
Biological Process:  anaphase-promoting complex-dependent catabolic process; cell cycle; cell division; cell migration; cell morphogenesis; cell projection organization; COPII vesicle coating; embryonic cleavage; endoplasmic reticulum to Golgi vesicle-mediated transport; fibroblast apoptotic process; gastrulation; gene expression; in utero embryonic development; inflammatory response; integrin-mediated signaling pathway; liver morphogenesis; mitotic cell cycle; mitotic metaphase plate congression; negative regulation of Rho protein signal transduction; negative regulation of transcription by RNA polymerase II; negative regulation of type I interferon production; nuclear protein quality control by the ubiquitin-proteasome system; positive regulation of cytokinesis; positive regulation of mitotic cell cycle phase transition; positive regulation of mitotic metaphase/anaphase transition; positive regulation of protein ubiquitination; proteasome-mediated ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein catabolic process; protein destabilization; protein K48-linked ubiquitination; protein monoubiquitination; protein polyubiquitination; protein ubiquitination; regulation of transcription by RNA polymerase II; regulation protein catabolic process at postsynapse; stem cell division; stress fiber assembly; trophectodermal cellular morphogenesis; ubiquitin-dependent protein catabolic process; vesicle-mediated transport; Wnt signaling pathway
Reference #:  Q9JLV5 (UniProtKB)
Alt. Names/Synonyms: AI467304; AW146203; CUL-3; Cul3; cullin 3; Cullin-3; KIAA0617; mKIAA0617
Gene Symbols: Cul3
Molecular weight: 88,948 Da
Basal Isoelectric point: 8.65  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  Reactome  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein