EPS8 Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes. Belongs to the EPS8 family. Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Chromosomal Location of mouse Ortholog: 6 G1|6 66.78 cM
Cellular Component:  brush border; cell cortex; cell projection; cytoplasm; cytosol; glutamatergic synapse; membrane; neuron projection; NMDA selective glutamate receptor complex; plasma membrane; postsynaptic density; ruffle membrane; stereocilium; stereocilium bundle; stereocilium tip; synapse
Molecular Function:  actin binding; protein binding; small GTPase binding
Biological Process:  actin crosslink formation; actin cytoskeleton organization; actin filament bundle assembly; actin polymerization-dependent cell motility; adult locomotory behavior; barbed-end actin filament capping; behavioral response to ethanol; cellular response to leukemia inhibitory factor; dendritic cell migration; exit from mitosis; positive regulation of ruffle assembly; Rac protein signal transduction; regulation of actin filament length; regulation of cell shape; regulation of postsynaptic membrane neurotransmitter receptor levels; regulation of Rho protein signal transduction; Rho protein signal transduction
Reference #:  Q08509 (UniProtKB)
Alt. Names/Synonyms: AW261790; Epidermal growth factor receptor kinase substrate 8; epidermal growth factor receptor pathway substrate 8; Eps8; OTTMUSP00000026105; OTTMUSP00000026106; OTTMUSP00000026108
Gene Symbols: Eps8
Molecular weight: 91,737 Da
Basal Isoelectric point: 7.32  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein