GAPDH Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC; Oxidoreductase
Chromosomal Location of Human Ortholog: 6 F2|6 59.32 cM
Cellular Component:  cytoplasm; cytosol; GAIT complex; glutamatergic synapse; intracellular membrane-bounded organelle; lipid droplet; microtubule cytoskeleton; nuclear membrane; nucleus; plasma membrane; postsynaptic density, intracellular component; ribonucleoprotein complex
Molecular Function:  aspartic-type endopeptidase inhibitor activity; disordered domain specific binding; enzyme binding; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; identical protein binding; microtubule binding; peptidyl-cysteine S-nitrosylase activity; protein binding; protein homodimerization activity
Biological Process:  antimicrobial humoral immune response mediated by antimicrobial peptide; apoptotic process; cAMP-mediated signaling; carbohydrate metabolic process; cellular response to interferon-gamma; defense response to fungus; gluconeogenesis; glycolytic process; killing by host of symbiont cells; killing of cells of other organism; microtubule cytoskeleton organization; multicellular organism development; negative regulation of endopeptidase activity; negative regulation of translation; negative regulation of vascular associated smooth muscle cell apoptotic process; neuron apoptotic process; peptidyl-cysteine S-trans-nitrosylation; positive regulation by organism of apoptotic process in other organism involved in symbiotic interaction; positive regulation of cytokine secretion; protein homotetramerization; protein stabilization; response to ammonium ion
Reference #:  P16858 (UniProtKB)
Alt. Names/Synonyms: G3P; Gapd; GAPDH; Glyceraldehyde-3-phosphate dehydrogenase; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; Peptidyl-cysteine S-nitrosylase GAPDH
Gene Symbols: Gapdh
Molecular weight: 35,810 Da
Basal Isoelectric point: 8.44  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene