PFKL Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway. Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade E sub-subfamily. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC; Kinase, other
Chromosomal Location of mouse Ortholog: 10 C1|10 39.72 cM
Cellular Component:  6-phosphofructokinase complex; cytoplasm; cytosol; membrane
Molecular Function:  6-phosphofructokinase activity; AMP binding; ATP binding; catalytic activity; fructose binding; fructose-6-phosphate binding; identical protein binding; kinase activity; kinase binding; metal ion binding; monosaccharide binding; nucleotide binding; transferase activity
Biological Process:  canonical glycolysis; fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; glycolytic process; glycolytic process through fructose-6-phosphate; metabolic process; negative regulation of insulin secretion; phosphorylation; response to glucose
Reference #:  P12382 (UniProtKB)
Alt. Names/Synonyms: 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; AA407869; ATP-dependent 6-phosphofructokinase, liver type; ATP-PFK; K6PL; OTTMUSP00000018445; PFK-B; Pfk-l; PFKAL; Pfkb; Pfkl; Phosphofructo-1-kinase isozyme B; Phosphofructokinase 1; phosphofructokinase, liver, B-type; Phosphohexokinase
Gene Symbols: Pfkl
Molecular weight: 85,360 Da
Basal Isoelectric point: 6.74  Predict pI for various phosphorylation states
CST Pathways:  Warburg Effect
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  Reactome  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein