NAA50 N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine. Has a broad substrate specificity: able to acetylate the initiator methionine of most peptides, except for those with a proline in second position. Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins. Autoacetylates in vivo. The relevance of N-epsilon-acetyltransferase activity is however unclear: able to acetylate H4 in vitro, but this result has not been confirmed in vivo. Component of a N-alpha-acetyltransferase complex containing NAA10 and NAA15, but NAA50 does not influence the acetyltransferase activity of NAA10: this multiprotein complex probably constitutes the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides. Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin: may act by counteracting the function of NAA10. Belongs to the acetyltransferase family. GNAT subfamily. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Acetyltransferase; EC 2.3.1.-
Chromosomal Location of human Ortholog: 3q13.31
Cellular Component:  cytoplasm; cytosol; NatA complex; nucleolus; nucleus
Molecular Function:  histone H4 acetyltransferase activity; N-acetyltransferase activity; peptide alpha-N-acetyltransferase activity; peptidyl-lysine acetyltransferase activity; protein binding
Biological Process:  establishment of mitotic sister chromatid cohesion; histone acetylation; mitotic sister chromatid cohesion; mitotic sister chromatid cohesion, centromeric; N-terminal protein amino acid acetylation
Reference #:  Q9GZZ1 (UniProtKB)
Alt. Names/Synonyms: FLJ13194; hNaa50p; hNAT5; hSAN; MAK3; N(alpha)-acetyltransferase 50, NatE catalytic subunit; N-acetyltransferase 13; N-acetyltransferase 13 (GCN5-related); N-acetyltransferase 5; N-acetyltransferase san homolog; N-alpha-acetyltransferase 50; N-alpha-acetyltransferase 50, NatE catalytic subunit; N-epsilon-acetyltransferase 50; NAA50; NAT13; NAT13P; NAT5; NAT5P; NatE catalytic subunit; SAN; separation anxiety
Gene Symbols: NAA50
Molecular weight: 19,398 Da
Basal Isoelectric point: 8.96  Predict pI for various phosphorylation states
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein