GAPDH Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC; Oxidoreductase
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component:  cytoplasm; cytosol; GAIT complex; intracellular membrane-bounded organelle; lipid droplet; microtubule cytoskeleton; nuclear membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; ribonucleoprotein complex
Molecular Function:  aspartic-type endopeptidase inhibitor activity; disordered domain specific binding; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; identical protein binding; microtubule binding; NAD binding; NADP binding; peptidyl-cysteine S-nitrosylase activity; protein binding
Biological Process:  antimicrobial humoral immune response mediated by antimicrobial peptide; canonical glycolysis; cellular response to interferon-gamma; defense response to fungus; gluconeogenesis; killing by host of symbiont cells; killing of cells of other organism; microtubule cytoskeleton organization; negative regulation of endopeptidase activity; negative regulation of translation; neuron apoptotic process; peptidyl-cysteine S-trans-nitrosylation; positive regulation by organism of apoptotic process in other organism involved in symbiotic interaction; positive regulation of cytokine secretion; protein stabilization; regulation of macroautophagy
Reference #:  P04406 (UniProtKB)
Alt. Names/Synonyms: aging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685
Gene Symbols: GAPDH
Molecular weight: 36,053 Da
Basal Isoelectric point: 8.57  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

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