CFIM25 Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. Belongs to the Nudix hydrolase family. CPSF5 subfamily. Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Note: This description may include information from UniProtKB.
Protein type: RNA splicing; RNA-binding; Spliceosome
Chromosomal Location of Human Ortholog: 16q13
Cellular Component:  centrosome; cytoplasm; microtubule organizing center; mRNA cleavage and polyadenylation specificity factor complex; mRNA cleavage factor complex; nuclear body; nucleoplasm; nucleus; paraspeckles
Molecular Function:  chromatin binding; histone deacetylase binding; hydrolase activity; identical protein binding; mRNA 3'-UTR AU-rich region binding; mRNA binding; protein binding; protein homodimerization activity
Biological Process:  cell differentiation; messenger ribonucleoprotein complex assembly; mRNA 3'-end processing; mRNA alternative polyadenylation; mRNA polyadenylation; mRNA processing; mRNA splicing, via spliceosome; positive regulation of mRNA cleavage; positive regulation of mRNA polyadenylation; positive regulation of pro-B cell differentiation; positive regulation of stem cell differentiation; posttranscriptional regulation of gene expression; pre-mRNA cleavage required for polyadenylation; protein heterotetramerization; protein tetramerization; termination of RNA polymerase II transcription
Reference #:  O43809 (UniProtKB)
Alt. Names/Synonyms: CFIM25; cleavage and polyadenylation specific factor 5, 25 kD subunit; cleavage and polyadenylation specific factor 5, 25 kDa; Cleavage and polyadenylation specificity factor 25 kDa subunit; Cleavage and polyadenylation specificity factor subunit 5; Cleavage factor Im complex 25 kDa subunit; CPSF 25 kDa subunit; CPSF25; CPSF5; DKFZp686H1588; Nucleoside diphosphate-linked moiety X motif 21; nudix (nucleoside diphosphate linked moiety X)-type motif 21; Nudix hydrolase 21; Nudix motif 21; NUDT21; pre-mRNA cleavage factor Im (25kD); Pre-mRNA cleavage factor Im 25 kDa subunit; Pre-mRNA cleavage factor Im 68 kDa subunit; pre-mRNA cleavage factor Im, 25kD subunit
Gene Symbols: NUDT21
Molecular weight: 26,227 Da
Basal Isoelectric point: 8.85  Predict pI for various phosphorylation states
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Protein Structure Not Found.

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