FAK Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription. Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous. 7 human isoforms generated by alternative promoter usage or alternative splicing have been reported. Note: This description may include information from UniProtKB.
Protein type: EC; Fak family; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group
Chromosomal Location of mouse Ortholog: 15 D3|15 33.94 cM
Cellular Component:  anchoring junction; apical plasma membrane; basolateral plasma membrane; cell projection; ciliary basal body; cytoplasm; cytoskeleton; cytosol; dendritic spine; focal adhesion; glutamatergic synapse; intercalated disc; intracellular membrane-bounded organelle; lamellipodium; membrane; nuclear body; nucleus; plasma membrane; postsynapse; sarcolemma; stress fiber
Molecular Function:  actin binding; ATP binding; identical protein binding; integrin binding; JUN kinase binding; kinase activity; molecular function activator activity; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphatase binding; phosphatidylinositol 3-kinase binding; protein binding; protein kinase activity; protein kinase binding; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine phosphatase activity; protein-containing complex binding; SH2 domain binding; transferase activity
Biological Process:  angiogenesis; axonogenesis; blood vessel development; cell migration; cellular response to transforming growth factor beta stimulus; central nervous system neuron axonogenesis; endothelial cell migration; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; extracellular matrix organization; growth hormone receptor signaling pathway; integrin-mediated signaling pathway; intracellular chloride ion homeostasis; JNK cascade; MAPK cascade; microtubule cytoskeleton organization; multicellular organism development; negative regulation of anoikis; negative regulation of apoptotic process; negative regulation of autophagy; negative regulation of axonogenesis; negative regulation of cell migration; negative regulation of cell-cell adhesion; negative regulation of organ growth; negative regulation of synapse assembly; neuron migration; nuclear migration; organ growth; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; phosphorylation; positive regulation of cardiac muscle hypertrophy; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell population proliferation; positive regulation of epithelial cell migration; positive regulation of epithelial to mesenchymal transition; positive regulation of fibroblast migration; positive regulation of glial cell proliferation; positive regulation of macrophage chemotaxis; positive regulation of macrophage proliferation; positive regulation of phagocytosis; positive regulation of phosphatidylinositol 3-kinase signaling; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of synaptic transmission; positive regulation of ubiquitin-dependent protein catabolic process; positive regulation of wound healing; protein autophosphorylation; protein phosphorylation; regulation of cell adhesion; regulation of cell adhesion mediated by integrin; regulation of cell population proliferation; regulation of cell shape; regulation of epithelial cell migration; regulation of focal adhesion assembly; regulation of modification of postsynaptic actin cytoskeleton; regulation of osteoblast differentiation; regulation of protein phosphorylation; regulation of substrate adhesion-dependent cell spreading; signal complex assembly; transforming growth factor beta receptor signaling pathway; vasculogenesis; vasodilation
Reference #:  P34152 (UniProtKB)
Alt. Names/Synonyms: Fad; Fadk; FADK 1; Fak; Fak1; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; Protein-tyrosine kinase 2; Ptk2; PTK2 protein tyrosine kinase 2
Gene Symbols: Ptk2
Molecular weight: 119,243 Da
Basal Isoelectric point: 6.19  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Angiogenesis  |  ErbB/HER Signaling  |  GPCR Signaling to MAPKs  |  Growth And Differentiation Control by MAPKs  |  PI3K/Akt Signaling  |  Tyrosine Kinases & Substrates
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Cross-references to other databases:  AlphaFold  |  BioGPS  |  Pfam  |  RCSB PDB  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein