CDC34 Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes. Belongs to the ubiquitin-conjugating enzyme family. Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription. Note: This description may include information from UniProtKB.
Protein type: EC; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 10 C1|10 39.72 cM
Cellular Component:  cytoplasm; cytosol; nuclear speck; nucleus
Molecular Function:  ATP binding; nucleotide binding; transferase activity; ubiquitin conjugating enzyme activity; ubiquitin-protein transferase activity
Biological Process:  cell cycle; cellular response to interferon-beta; negative regulation of cAMP-mediated signaling; positive regulation of inclusion body assembly; positive regulation of neuron apoptotic process; proteasome-mediated ubiquitin-dependent protein catabolic process; protein K48-linked ubiquitination; protein polyubiquitination; protein ubiquitination; ubiquitin-dependent protein catabolic process
Reference #:  Q8CFI2 (UniProtKB)
Alt. Names/Synonyms: (E3-independent) E2 ubiquitin-conjugating enzyme R1; AI327276; Cdc34; cell division cycle 34; cell division cycle 34 homolog; cell division cycle 34 homolog (S. cerevisiae); E2 ubiquitin-conjugating enzyme R1; E2-CDC34; UB2R1; Ubch3; UBE2R; Ube2r1; ubiquitin-conjugating enzyme Cdc34; Ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
Gene Symbols: Cdc34
Molecular weight: 26,622 Da
Basal Isoelectric point: 4.44  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein