Pnk1 Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone. In the N-terminal section; belongs to the DNA 3' phosphatase family. Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193). 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: DNA-binding; EC; EC; Kinase, other; Phosphatase (non-protein)
Chromosomal Location of mouse Ortholog: 7|7 B3
Cellular Component:  mitochondrion; nucleolus; nucleus
Molecular Function:  ATP binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; catalytic activity; double-stranded DNA binding; hydrolase activity; kinase activity; nucleoside monophosphate kinase activity; nucleotide binding; polynucleotide 3'-phosphatase activity; transferase activity
Biological Process:  cellular response to DNA damage stimulus; dephosphorylation; DNA 3' dephosphorylation involved in DNA repair; DNA damage response, detection of DNA damage; DNA repair; metabolic process; negative regulation of protein ADP-ribosylation; nucleotide phosphorylation; phosphorylation; positive regulation of telomerase activity; positive regulation of telomere capping; positive regulation of telomere maintenance via telomerase; response to oxidative stress
Reference #:  Q9JLV6 (UniProtKB)
Alt. Names/Synonyms: 1810009G08Rik; 2'(3')-polynucleotidase; Bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; OTTMUSP00000025291; OTTMUSP00000025292; PNK; Pnk1; Pnkp; Polynucleotide 3'-phosphatase; Polynucleotide 5'-hydroxyl-kinase; polynucleotide kinase 3'- phosphatase; Polynucleotide kinase-3'-phosphatase
Gene Symbols: Pnkp
Molecular weight: 57,223 Da
Basal Isoelectric point: 8.03  Predict pI for various phosphorylation states
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene