Pnk1 Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone. In the N-terminal section; belongs to the DNA 3' phosphatase family. Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193). 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: DNA-binding; EC; EC; Kinase, other; Phosphatase (non-protein)
Chromosomal Location of human Ortholog: 19q13.33
Cellular Component:  mitochondrion; nucleolus; nucleoplasm; nucleus
Molecular Function:  ATP binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; damaged DNA binding; double-stranded DNA binding; endonuclease activity; nucleoside monophosphate kinase activity; polynucleotide 3'-phosphatase activity; protein binding; purine nucleotide binding
Biological Process:  dephosphorylation; DNA damage response, detection of DNA damage; DNA repair; DNA-dependent DNA replication; negative regulation of protein ADP-ribosylation; nucleic acid phosphodiester bond hydrolysis; nucleoside monophosphate phosphorylation; nucleotide phosphorylation; nucleotide-excision repair, DNA damage removal; polynucleotide 3' dephosphorylation; positive regulation of telomerase activity; positive regulation of telomere capping; positive regulation of telomere maintenance via telomerase; response to oxidative stress; response to radiation
Disease: Ataxia-oculomotor Apraxia 4; Charcot-marie-tooth Disease, Axonal, Type 2b2; Microcephaly, Seizures, And Developmental Delay
Reference #:  Q96T60 (UniProtKB)
Alt. Names/Synonyms: 2'(3')-polynucleotidase; AOA4; Bifunctional polynucleotide phosphatase/kinase; CMT2B2; DNA 5'-kinase/3'-phosphatase; EIEE10; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP); MCSZ; PNK; Pnk1; PNKP; Polynucleotide 3'-phosphatase; Polynucleotide 5'-hydroxyl-kinase; polynucleotide kinase 3'-phosphatase; polynucleotide kinase 3-prime-phosphatase; Polynucleotide kinase-3'-phosphatase
Gene Symbols: PNKP
Molecular weight: 57,076 Da
Basal Isoelectric point: 8.73  Predict pI for various phosphorylation states
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene