PSF a DNA- and RNA-binding protein. Is involved in several nuclear processes including splicing and transcriptional regulation. Is an essential pre-mRNA splicing factor required early in spliceosome formation. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. In vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. In vivo, it binds directly to the DNA substrates and cooperates with the Ku70/Ku80 dimer to establish a functional preligation complex. Is involved in transcriptional regulation. Transcriptional repression is mediated by an interaction with SIN3A and subsequent recruitment of histone deacetylases (HDACs). Involved in the regulation of basal and cAMP-dependent transcriptional activity. Binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds DNA in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK/BMAL1 heterodimer. Required for the assembly of nuclear speckles. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; RNA splicing; RNA-binding
Chromosomal Location of human Ortholog: 1p34.3
Cellular Component:  chromatin; cytosol; nuclear matrix; nuclear speck; nucleoplasm; nucleus; paraspeckles; RNA polymerase II transcription regulator complex
Molecular Function:  chromatin binding; DNA binding; histone deacetylase binding; protein binding; protein homodimerization activity; RNA binding; transcription cis-regulatory region binding
Biological Process:  activation of innate immune response; alternative mRNA splicing, via spliceosome; chromatin remodeling; double-strand break repair via homologous recombination; innate immune response; mRNA processing; negative regulation of circadian rhythm; negative regulation of DNA-templated transcription; negative regulation of transcription by RNA polymerase II; positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway; positive regulation of transcription by RNA polymerase II; regulation of circadian rhythm; regulation of DNA-templated transcription; rhythmic process; RNA splicing
Reference #:  P23246 (UniProtKB)
Alt. Names/Synonyms: 100 kDa DNA-pairing protein; DNA-binding p52/p100 complex, 100 kDa subunit; epididymis secretory sperm binding protein; hPOMp100; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; Polypyrimidine tract-binding protein-associated-splicing factor; POMP100; PPP1R140; protein phosphatase 1, regulatory subunit 140; PSF; PTB-associated splicing factor; PTB-associated-splicing factor; SFPQ; splicing factor proline and glutamine rich; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); splicing factor proline/glutamine-rich; splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated); Splicing factor, proline- and glutamine-rich
Gene Symbols: SFPQ
Molecular weight: 76,149 Da
Basal Isoelectric point: 9.45  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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PSF

Protein Structure Not Found.


Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  NURSA  |  Ensembl Protein