USP16 Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. Belongs to the peptidase C19 family. USP16 subfamily. Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. 5 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease
Chromosomal Location of Human Ortholog: 16|16 C3.3
Cellular Component:  nucleus
Molecular Function:  cysteine-type endopeptidase activity; cysteine-type peptidase activity; histone binding; hydrolase activity; metal ion binding; peptidase activity; protein binding; thiol-dependent ubiquitin-specific protease activity; thiol-dependent ubiquitinyl hydrolase activity; transcription coactivator activity; ubiquitin binding; zinc ion binding
Biological Process:  cell cycle; cell division; cellular response to DNA damage stimulus; chromatin organization; histone deubiquitination; histone H2A K63-linked deubiquitination; mitotic cell cycle; monoubiquitinated histone H2A deubiquitination; positive regulation of transcription by RNA polymerase II; positive regulation of transcription, DNA-templated; positive regulation of translational elongation; protein deubiquitination; protein homotetramerization; proteolysis; regulation of cell cycle; regulation of gene expression; regulation of transcription by RNA polymerase II; ubiquitin-dependent protein catabolic process
Reference #:  Q99LG0 (UniProtKB)
Alt. Names/Synonyms: 1200004E02Rik; 2810483I07Rik; 6330514E22Rik; Deubiquitinating enzyme 16; Ubiquitin carboxyl-terminal hydrolase 16; ubiquitin specific peptidase 16; ubiquitin specific protease 16; Ubiquitin thioesterase 16; Ubiquitin-specific-processing protease 16; UBP16; UBPM; Usp16
Gene Symbols: Usp16
Molecular weight: 93,434 Da
Basal Isoelectric point: 5.83  Predict pI for various phosphorylation states
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  STRING  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene