IFI16 Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Belongs to the HIN-200 family. Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prostate, ovary and breast (at protein level). 4 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Transcription factor
Chromosomal Location of human Ortholog: 1q23.1
Cellular Component:  cytosol; nuclear speck; nucleolus; nucleoplasm; nucleus
Molecular Function:  DNA-binding transcription repressor activity, RNA polymerase II-specific; double-stranded DNA binding; protein binding; RNA polymerase II proximal promoter sequence-specific DNA binding; transcription factor binding
Biological Process:  activation of cysteine-type endopeptidase activity; activation of innate immune response; autophagy; cell proliferation; cellular response to glucose starvation; cellular response to interferon-beta; cellular response to ionizing radiation; defense response to virus; hemopoiesis; inflammatory response; innate immune response; intrinsic apoptotic signaling pathway by p53 class mediator; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; monocyte differentiation; myeloid cell differentiation; negative regulation of cysteine-type endopeptidase activity; negative regulation of DNA binding; negative regulation of innate immune response; negative regulation of transcription by RNA polymerase II; negative regulation of transcription, DNA-templated; negative regulation of viral genome replication; positive regulation of cytokine production; positive regulation of interleukin-1 beta production; positive regulation of transcription by RNA polymerase II; positive regulation of type I interferon production; regulation of autophagy; regulation of gene expression, epigenetic
Reference #:  Q16666 (UniProtKB)
Alt. Names/Synonyms: Gamma-interferon-inducible protein 16; IF16; Ifi-16; IFI16; IFNGIP1; interferon gamma inducible protein 16; interferon, gamma-inducible protein 16; interferon-gamma induced protein IFI 16; Interferon-inducible myeloid differentiation transcriptional activator; MGC9466; PYHIN2
Gene Symbols: IFI16
Molecular weight: 88,256 Da
Basal Isoelectric point: 9.31  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

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