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RFFL
E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling. Ubiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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| Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase; Vesicle |
| Chromosomal Location of rat Ortholog: 10q26 |
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Cellular Component:
cytoplasm; cytoplasmic vesicle; cytosol; endosome membrane; Golgi membrane; lysosome; plasma membrane; recycling endosome membrane
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Molecular Function:
ATP binding; metal ion binding; p53 binding; protease binding; protein kinase binding; ubiquitin protein ligase activity; ubiquitin protein ligase binding
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Biological Process:
apoptotic process; intracellular protein transport; negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; negative regulation of signal transduction by p53 class mediator; negative regulation of tumor necrosis factor-mediated signaling pathway; proteasome-mediated ubiquitin-dependent protein catabolic process; protein K48-linked ubiquitination; regulation of fibroblast migration; ubiquitin-dependent protein catabolic process
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Reference #:
Q8CIN9
(UniProtKB)
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Alt. Names/Synonyms: E3 ubiquitin-protein ligase rififylin; RFFL
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Gene Symbols: Rffl
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Molecular weight:
40,409 Da
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Basal Isoelectric point:
5.41
Predict pI for various phosphorylation states
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