RTF1 Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single- stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex. Note: This description may include information from UniProtKB.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 15q15.1
Cellular Component:  Cdc73/Paf1 complex; nucleolus; nucleoplasm
Molecular Function:  protein binding; single-stranded DNA binding
Biological Process:  blastocyst growth; endodermal cell fate commitment; histone H3-K4 trimethylation; negative regulation of transcription by RNA polymerase II; positive regulation of histone H3-K4 methylation; positive regulation of transcription by RNA polymerase II; positive regulation of transcription elongation from RNA polymerase II promoter; protein ubiquitination; stem cell population maintenance; transcription by RNA polymerase II; transcription elongation from RNA polymerase II promoter; Wnt signaling pathway
Disease: Congenital Disorder Of Glycosylation, Type In
Reference #:  Q92541 (UniProtKB)
Alt. Names/Synonyms: CDG1N; GTL7; KIAA0252; ortholog of mouse gene trap locus 7; RNA polymerase-associated protein RTF1 homolog; RTF1; Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Gene Symbols: RTF1
Molecular weight: 80,313 Da
Basal Isoelectric point: 8.21  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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RTF1

Protein Structure Not Found.


Cross-references to other databases:  STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  InnateDB