GRF-1 Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity. This binding is inhibited by phosphorylation by PRKCA. Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis. Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation. Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation. During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth. Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences. Detected in neutrophils (at protein level). Note: This description may include information from UniProtKB.
Protein type: GAP; GAP, Rac/Rho; Motility/polarity/chemotaxis; Transcription factor; Tumor suppressor
Chromosomal Location of Human Ortholog: 1q21
Cellular Component:  actin cytoskeleton; ciliary basal body; cytoplasm; cytosol; nucleus; plasma membrane
Molecular Function:  DNA binding; DNA-binding transcription repressor activity, RNA polymerase II-specific; GTP binding; GTPase activating protein binding; GTPase activator activity; GTPase activity; phospholipid binding; protein-containing complex binding; RNA polymerase II regulatory region sequence-specific DNA binding
Biological Process:  axon guidance; axonal fasciculation; camera-type eye development; cell migration; cellular response to extracellular stimulus; central nervous system neuron axonogenesis; establishment or maintenance of actin cytoskeleton polarity; forebrain development; mammary gland development; negative regulation of Rho protein signal transduction; negative regulation of transcription by RNA polymerase II; negative regulation of transcription, DNA-templated; negative regulation of vascular permeability; neural tube closure; neuron projection guidance; positive regulation of cilium assembly; positive regulation of GTPase activity; positive regulation of neuron projection development; regulation of actin cytoskeleton organization; regulation of actin polymerization or depolymerization; regulation of axonogenesis; regulation of cell shape; signal transduction; wound healing, spreading of cells
Reference #:  P81128 (UniProtKB)
Alt. Names/Synonyms: GAP-associated protein p190; glucocorticoid receptor DNA binding factor 1; Glucocorticoid receptor DNA-binding factor 1; Grlf1; Rho GTPase-activating protein 35
Gene Symbols: Grlf1
Molecular weight: 172,379 Da
Basal Isoelectric point: 6.28  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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GRF-1

Protein Structure Not Found.


Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene