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G3BP-1
an hnRNA-binding protein and endoribonuclease that participates in the Ras signal transduction pathway. A regulated effector of stress granule (SG) assembly. SGs are involved in mRNA sorting in the storing of untranslated mRNAs. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Binds to the SH3 domain of Ras GTPase-activating protein (RasGAP) in proliferating cells but not in quiescent cells. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells. Recruited to SGs upon either arsenite or high temperature treatment. RasGAP-dependent phosphorylation of S149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. S149 phosphorylation induces partial nuclear localization. A component of a TAU mRNP complex, Interacts with USP10, and may regulate it. Note: This description may include information from UniProtKB.
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| Protein type: Adaptor/scaffold; EC 3.6.4.12; EC 3.6.4.13; Helicase; RNA-binding |
| Chromosomal Location of Human Ortholog: 5q33.1 |
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Cellular Component:
cytoplasm; cytoplasmic stress granule; cytosol; nucleus; perikaryon
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Molecular Function:
ATP binding; ATP-dependent DNA helicase activity; ATP-dependent RNA helicase activity; DNA binding; endonuclease activity; protein binding
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Biological Process:
defense response to virus; DNA duplex unwinding; negative regulation of canonical Wnt signaling pathway; nucleic acid phosphodiester bond hydrolysis; positive regulation of stress granule assembly; Ras protein signal transduction; stress granule assembly; type I interferon production; viral process
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Reference #:
Q13283
(UniProtKB)
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Alt. Names/Synonyms: ATP-dependent DNA helicase VIII; G3BP; G3BP-1; G3BP1; GAP binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP
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Gene Symbols: G3BP1
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Molecular weight:
52,164 Da
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Basal Isoelectric point:
5.36
Predict pI for various phosphorylation states
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Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®
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