MST2 Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Autophagy; EC; Kinase, protein; MST subfamily; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family
Chromosomal Location of mouse Ortholog: 15|15 B3.1
Cellular Component:  cytoplasm; nucleus; protein-containing complex
Molecular Function:  ATP binding; identical protein binding; kinase activity; magnesium ion binding; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity
Biological Process:  activation of protein kinase activity; apoptotic process; cell differentiation involved in embryonic placenta development; central nervous system development; endocardium development; hepatocyte apoptotic process; hippo signaling; intracellular signal transduction; negative regulation of canonical Wnt signaling pathway; negative regulation of cell proliferation; negative regulation of organ growth; neural tube formation; phosphorylation; positive regulation of apoptotic process; positive regulation of DNA-binding transcription factor activity; positive regulation of extrinsic apoptotic signaling pathway via death domain receptors; positive regulation of fat cell differentiation; positive regulation of JNK cascade; positive regulation of protein binding; positive regulation of protein kinase B signaling; primitive hemopoiesis; protein phosphorylation; protein stabilization; regulation of cell differentiation involved in embryonic placenta development; signal transduction; signal transduction by protein phosphorylation; stress-activated protein kinase signaling cascade
Reference #:  Q9JI10 (UniProtKB)
Alt. Names/Synonyms: 0610042I06Rik; Mammalian STE20-like protein kinase 2; mes; Mess1; MST; MST-2; Mst2; MST2/C; MST2/N; Mst3; protein kinase homolog; serine/threonine kinase 3; serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine-protein kinase 3; Serine/threonine-protein kinase 3 20kDa subunit; Serine/threonine-protein kinase 3 36kDa subunit; Ste2; Ste20; STE20-like kinase MST2; Stk3
Gene Symbols: Stk3
Molecular weight: 56,855 Da
Basal Isoelectric point: 4.9  Predict pI for various phosphorylation states
CST Pathways:  Hippo Signaling
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
Select Structure to View Below


Protein Structure Not Found.

Substrate Sequence Logo
Open Substrate Page

Cross-references to other databases:  AlphaFold  |  STRING  |  Reactome  |  BioGPS  |  KinBase  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene