NANOG
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. Belongs to the Nanog homeobox family. Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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Protein type: Cell development/differentiation; DNA-binding; Transcription factor |
Chromosomal Location of human Ortholog: 12p13.31 |
Cellular Component:
chromatin; intracellular membrane-bounded organelle; nucleolus; nucleoplasm; nucleus
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Molecular Function:
DNA binding; DNA-binding transcription factor activity; DNA-binding transcription factor activity, RNA polymerase II-specific; DNA-binding transcription repressor activity, RNA polymerase II-specific; protein binding; RNA polymerase II cis-regulatory region sequence-specific DNA binding; RNA polymerase II transcription regulatory region sequence-specific DNA binding; sequence-specific double-stranded DNA binding; transcription cis-regulatory region binding
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Biological Process:
cell differentiation; endodermal cell fate specification; negative regulation of transcription by RNA polymerase II; positive regulation of stem cell proliferation; positive regulation of transcription by RNA polymerase II; regulation of cell differentiation; regulation of DNA-templated transcription; regulation of gene expression; regulation of transcription by RNA polymerase II; somatic stem cell population maintenance; stem cell population maintenance
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Reference #:
Q9H9S0
(UniProtKB)
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Alt. Names/Synonyms: hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox
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Gene Symbols: NANOG
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Molecular weight:
34,620 Da
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Basal Isoelectric point:
6.32
Predict pI for various phosphorylation states
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CST Pathways:
ESC Pluripotency and Differentiation
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Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology®
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