AP2M1 Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1. Plays a role in endocytosis of frizzled family members upon Wnt signaling. Belongs to the adaptor complexes medium subunit family. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold; Vesicle
Chromosomal Location of Human Ortholog: 3q27.1
Cellular Component:  AP-2 adaptor complex; clathrin-coated endocytic vesicle; clathrin-coated endocytic vesicle membrane; cytosol; endocytic vesicle membrane; endolysosome membrane; lysosomal membrane; plasma membrane
Molecular Function:  ion channel binding; lipid binding; low-density lipoprotein particle receptor binding; protein binding; signal sequence binding
Biological Process:  antigen processing and presentation of exogenous peptide antigen via MHC class II; cellular protein-containing complex assembly; clathrin-dependent endocytosis; ephrin receptor signaling pathway; intracellular protein transport; low-density lipoprotein particle clearance; low-density lipoprotein particle receptor catabolic process; membrane organization; negative regulation of protein localization to plasma membrane; receptor internalization; regulation of defense response to virus by virus; regulation of vesicle size; vesicle budding from membrane; Wnt signaling pathway, planar cell polarity pathway
Disease: Intellectual Developmental Disorder 60 With Seizures
Reference #:  Q96CW1 (UniProtKB)
Alt. Names/Synonyms: Adapter-related protein complex 2 mu subunit; Adaptin-mu2; Adaptor protein complex AP-2 subunit mu; adaptor-related protein complex 2, mu 1 subunit; AP-2 complex subunit mu; AP-2 mu 2 chain; AP-2 mu chain; AP-2 mu2; AP2M1; AP50; CLAPM1; clathrin adaptor complex AP2, mu subunit; Clathrin assembly protein complex 2 medium chain; clathrin coat adaptor protein AP50; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; clathrin-associated/assembly/adaptor protein, medium 1; HA2 50 kDa subunit; KIAA0109; mu2; Plasma membrane adaptor AP-2 50 kDa protein; plasma membrane adaptor AP-2 50kDA protein
Gene Symbols: AP2M1
Molecular weight: 49,655 Da
Basal Isoelectric point: 9.57  Predict pI for various phosphorylation states
Select Structure to View Below

AP2M1

Protein Structure Not Found.


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