PACT Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner. Belongs to the PRKRA family. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Activator; RNA processing; RNA-binding
Chromosomal Location of rat Ortholog: 3q24
Cellular Component:  cytoplasm; cytosol; nucleoplasm; perinuclear region of cytoplasm; RISC-loading complex
Molecular Function:  double-stranded RNA binding; enzyme activator activity; enzyme binding; identical protein binding; kinase activity; pre-miRNA binding; protein homodimerization activity; protein kinase binding
Biological Process:  cellular response to oxidative stress; ear development; middle ear morphogenesis; outer ear morphogenesis; positive regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of intrinsic apoptotic signaling pathway; pre-miRNA processing; production of miRNAs involved in gene silencing by miRNA; production of siRNA involved in RNA interference; protein phosphorylation; protein stabilization; skeletal system morphogenesis
Reference #:  Q4V8C7 (UniProtKB)
Alt. Names/Synonyms: Interferon-inducible double stranded RNA-dependent protein kinase activator A; Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PACT; Prkra; Protein activator of the interferon-induced protein kinase; protein kinase, interferon inducible double stranded RNA dependent activator; Protein kinase, interferon-inducible double stranded RNA-dependent activator
Gene Symbols: Prkra
Molecular weight: 34,355 Da
Basal Isoelectric point: 8.81  Predict pI for various phosphorylation states
Select Structure to View Below


Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  Reactome  |  BioGPS  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene