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PACT
Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner. Belongs to the PRKRA family. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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| Protein type: Activator; RNA processing; RNA-binding |
| Chromosomal Location of rat Ortholog: 3q24 |
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Cellular Component:
cytoplasm; cytosol; nucleoplasm; perinuclear region of cytoplasm; RISC-loading complex
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Molecular Function:
double-stranded RNA binding; enzyme activator activity; enzyme binding; identical protein binding; kinase activity; pre-miRNA binding; protein homodimerization activity; protein kinase binding
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Biological Process:
cellular response to oxidative stress; ear development; middle ear morphogenesis; outer ear morphogenesis; positive regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of intrinsic apoptotic signaling pathway; pre-miRNA processing; production of miRNAs involved in gene silencing by miRNA; production of siRNA involved in RNA interference; protein phosphorylation; protein stabilization; skeletal system morphogenesis
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Reference #:
Q4V8C7
(UniProtKB)
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Alt. Names/Synonyms: Interferon-inducible double stranded RNA-dependent protein kinase activator A; Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PACT; Prkra; Protein activator of the interferon-induced protein kinase; protein kinase, interferon inducible double stranded RNA dependent activator; Protein kinase, interferon-inducible double stranded RNA-dependent activator
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Gene Symbols: Prkra
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Molecular weight:
34,355 Da
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Basal Isoelectric point:
8.81
Predict pI for various phosphorylation states
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