BRD4 Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6. BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo. In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters. Isoform B: Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AFX/H2A.x phosphorylation. Ubiquitously expressed. 3 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: ATYPICAL group; BRD family; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical
Chromosomal Location of Human Ortholog: 17 B1|17 17.39 cM
Cellular Component:  chromosome; condensed nuclear chromosome; cyclin/CDK positive transcription elongation factor complex; nuclear chromatin; nucleus
Molecular Function:  chromatin binding; DNA binding; GTP-dependent protein kinase activity; protein binding; RNA polymerase II C-terminal domain binding; RNA polymerase II CTD heptapeptide repeat kinase activity
Biological Process:  chromatin organization; chromosome segregation; histone H3-K14 acetylation; histone H4-K12 acetylation; inner cell mass cell proliferation; positive regulation of DNA binding; positive regulation of G2/M transition of mitotic cell cycle; positive regulation of transcription elongation from RNA polymerase II promoter; protein autophosphorylation; protein phosphorylation; regulation of transcription involved in G1/S transition of mitotic cell cycle
Reference #:  Q9ESU6 (UniProtKB)
Alt. Names/Synonyms: Brd4; Brd5; bromodomain containing 4; bromodomain-containing 5; Bromodomain-containing protein 4; fsh-like protein; HUNK; HUNK1; MCAP; Mitotic chromosome-associated protein; WI-1151; WI-11513
Gene Symbols: Brd4
Molecular weight: 155,895 Da
Basal Isoelectric point: 9.23  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
Select Structure to View Below


Protein Structure Not Found.